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KMID : 0360419940300010111
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Korean Journal of Pharmacology
1994 Volume.30 No. 1 p.111 ~ p.116
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The Role of /Calmodulin-Dependent Protein Kinase II on the Norepinephrine and GTP-Increased Myosin tight Chain Phosphorylations in Rabbit Mesenteric Permeabilized Artery
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Ahn Hee-Yul
Kim Hun-Sik
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Abstract
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The role of /calmodulin-dependent protein kinase II in the increase of myofilament sensitivity by agonist and GTP was investigated in rabbit mesenteric permeabilized artery. increased myosin light chain phosphorylations monotonically. norepinephrine and GTP potentiated increase of myosin light chain phosphorylations by , which reaches a peak at 5 min and gradually declines to the alone level at 20 min. At the early phase (1 min), KN 62, the inhibitor of /calmodulin-dependent protein kinase II , decreased myosin light chain phosphorylation levels by norepinephrine and GTP in the presence of KN-62 did not affect the myosin light chain phosphorylations by norepinephrine and GTP in the presence of at the peak (5 min) and plateau phases (20 min). From these results, the role of /calmodulin-dependent protein kinase II may be different depending on time, which may play a role in increase of myofilamint sensitivity by norepinephrine and GTP resulting from increase of myosin light chain phosphorylations at the early phase. However, at plateau phase, /calmodulin-dependent protein kinase II may not be involved in the increase of myofilament sensitivity by norepinephrine and GTP in rabbit mesenteric permeabilized artery.
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KEYWORD
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KN-62, -Calmodulin dependent protein kinase ll, and Myosin light chain phosphorylations
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